Cation transport and specificity of ionomycin. Comparison with ionophore A23187 in rat liver mitochondria.
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منابع مشابه
Cation transport and specificity of ionomycin. Comparison with ionophore A23187 in rat liver mitochondria.
Based on the effects of ionomycin upon mitochondrial respiration, ionomycin was shown to be an effective ionophore for Ca2+ in rat liver mitochondria. The ionomycin-induced efflux of Ca2+ across the inner membrane was more sensitive to loading the mitochondria with Ca2+ than was efflux catalyzed by A23187. At saturating concentrations of Ca2+, the turnover number for ionomycin was 3- to 5-fold ...
متن کاملThe specificity of ionophore A23187 in cation transport across lipid membranes. Studies with lecithin vesicles.
Lecithin vesicles containing different cations were prepared by sonication and characterized. When the concentration of the divalent cations in the buffer was increased from 1 mM to about 100 mM the cation-concentration inside of the vesicles did not increase proportional to the outside concentration, but showed a saturation behaviour. The efflux of various cations mediated by ionophore A23187 ...
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A23187 is a carboxylic acid antibiotic which simultaneously uncouples oxidative phosphorylation and inhibits ATPase of rat liver mitochondria incubated in a magnesium-free medium. The antibiotic acts as a freely mobile carrier to transport calcium and magnesium, but not potassium, from an aqueous medium buffered at pH 7.4 into a bulk organic phase. A23187 causes a progressive release of endogen...
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Vesicles have been prepared by mild sonication of a purified inner membrane fraction from rat liver mitochondria. Such vesicles demonstrate an energy-dependent net concentrative uptake of inorganic phosphate in the absence of added Na+, Ca’+, or ionophores. Phosphate uptake can be supported by a variety of substrates including /?-hydroxybutyrate, succinate, and ascorbate + tetramethylphenylened...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1980
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)85799-x